EVALUATION IN VITRO CYTOTOXICITY OF DIFFERENT OXYGEN DOPING ON THE TI-20MO ALLOY

Referencia Apresentador Autores
(Instituição)
Resumo
02-024
Tatiani Ayako Goto Donato Capra, A.A.(Universidade Estadual Paulista); Nogueira, R.A.(Universidade Estadual Paulista); Araújo, R.O.(UNESP - Universidade Estadual Paulista); Grandini, C.R.(Universidade Estadual Paulista); Donato, T.A.(Universidade Estadual Paulista); Titanium (Ti) is the consensual biomaterial employed in implants for allowing a well osseointegration. Recently, promising alloys that added niobium, tantalum, zirconium and molybdenum are being investigated. To investigate the biocompatibility as well as the differentiation of osteoblastic cells cultived of a Ti-20Mo alloy after annealing heat treatment comparing with Ti-20Mo alloy doped with different oxygen using in vitro cell viability, immunofluorescense and alkaline phosphatase. For this, MC3T3-E1 line cells were cultured on Ti-20Mo alloys with base medium. These cells were exposed on Ti-20Mo alloys for 48 hours to assay MTT, SEM and indirect immunofluorescence. The assay alkaline phosphatase was analyzed with 7 days. The present results demonstrated that all alloys studied presented no cytotoxic effects on the osteogenic cells. In addition, a high activity of alkaline phosphatase was observed. All of them, independently from the treatment, showed a central and flattened cell body and numerous and long processes.
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